UpaG, a New Member of the Trimeric Autotransporter Family of Adhesins in Uropathogenic Escherichia coli
Identifieur interne : 008945 ( Main/Exploration ); précédent : 008944; suivant : 008946UpaG, a New Member of the Trimeric Autotransporter Family of Adhesins in Uropathogenic Escherichia coli
Auteurs : Jaione Valle [France] ; Amanda N. Mabbett [Australie] ; Glen C. Ulett [Australie] ; Alejandro Toledo-Arana [France] ; Karine Wecker [France] ; Makrina Totsika [Australie] ; Mark A. Schembri [Australie] ; Jean-Marc Ghigo [France] ; Christophe Beloin [France]Source :
- Journal of bacteriology [ 0021-9193 ] ; 2008.
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- Pascal (Inist)
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Abstract
The ability of Escherichia coli to colonize both intestinal and extraintestinal sites is driven by the presence of specific virulence factors, among which are the autotransporter (AT) proteins. Members of the trimeric AT adhesin family are important virulence factors for several gram-negative pathogens and mediate adherence to eukaryotic cells and extracellular matrix (ECM) proteins. In this study, we characterized a new trimeric AT adhesin (UpaG) from uropathogenic E. coli (UPEC). Molecular analysis of UpaG revealed that it is translocated to the cell surface and adopts a multimeric conformation. We demonstrated that UpaG is able to promote cell aggregation and biofilm formation on abiotic surfaces in CFT073 and various UPEC strains. In addition, UpaG expression resulted in the adhesion of CFT073 to human bladder epithelial cells, with specific affinity to fibronectin and laminin. Prevalence analysis revealed that upaG is strongly associated with E. coli strains from the B2 and D phylogenetic groups, while deletion ofupaG had no significant effect on the ability of CFT073 to colonize the mouse urinary tract. Thus, UpaG is a novel trimeric AT adhesin from E. coli that mediates aggregation, biofilm formation, and adhesion to various ECM proteins.
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Ulett</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1><s2>Brisbane QLD 4072</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Brisbane QLD 4072</wicri:noRegion></affiliation></author><author><name sortKey="Toledo Arana, Alejandro" sort="Toledo Arana, Alejandro" uniqKey="Toledo Arana A" first="Alejandro" last="Toledo-Arana">Alejandro Toledo-Arana</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Institut Pasteur, Unité des Interactions Bactéries-Cellules, 25 Rue du Dr. Roux</s1><s2>75015 Paris</s2><s3>FRA</s3><sZ>4 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Wecker, Karine" sort="Wecker, Karine" uniqKey="Wecker K" first="Karine" last="Wecker">Karine Wecker</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Institut Pasteur, Unité de Résonance Magnétique Nucléaire des Biomolécules, CNRS URA 2185, 28 Rue du Dr. Roux</s1><s2>75015 Paris</s2><s3>FRA</s3><sZ>5 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Totsika, Makrina" sort="Totsika, Makrina" uniqKey="Totsika M" first="Makrina" last="Totsika">Makrina Totsika</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1><s2>Brisbane QLD 4072</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Brisbane QLD 4072</wicri:noRegion></affiliation></author><author><name sortKey="Schembri, Mark A" sort="Schembri, Mark A" uniqKey="Schembri M" first="Mark A." last="Schembri">Mark A. Schembri</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>School of Molecular and Microbial Sciences, University of Queensland</s1><s2>Brisbane QLD 4072</s2><s3>AUS</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Brisbane QLD 4072</wicri:noRegion></affiliation></author><author><name sortKey="Ghigo, Jean Marc" sort="Ghigo, Jean Marc" uniqKey="Ghigo J" first="Jean-Marc" last="Ghigo">Jean-Marc Ghigo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institut Pasteur, Unité de Génétique des Biofilms, CNRS URA 2172, 25 Rue du Dr. Roux</s1><s2>75015 Paris</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Beloin, Christophe" sort="Beloin, Christophe" uniqKey="Beloin C" first="Christophe" last="Beloin">Christophe Beloin</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institut Pasteur, Unité de Génétique des Biofilms, CNRS URA 2172, 25 Rue du Dr. Roux</s1><s2>75015 Paris</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>75015 Paris</wicri:noRegion><placeName><settlement type="city">Paris</settlement><region type="région" nuts="2">Île-de-France</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of bacteriology</title><title level="j" type="abbreviated">J. bacteriol.</title><idno type="ISSN">0021-9193</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of bacteriology</title><title level="j" type="abbreviated">J. bacteriol.</title><idno type="ISSN">0021-9193</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adhesin</term><term>Escherichia coli</term><term>Microbiology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Escherichia coli</term><term>Adhésine</term><term>Microbiologie</term><term>Souche uropathogène</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The ability of Escherichia coli to colonize both intestinal and extraintestinal sites is driven by the presence of specific virulence factors, among which are the autotransporter (AT) proteins. Members of the trimeric AT adhesin family are important virulence factors for several gram-negative pathogens and mediate adherence to eukaryotic cells and extracellular matrix (ECM) proteins. In this study, we characterized a new trimeric AT adhesin (UpaG) from uropathogenic E. coli (UPEC). Molecular analysis of UpaG revealed that it is translocated to the cell surface and adopts a multimeric conformation. We demonstrated that UpaG is able to promote cell aggregation and biofilm formation on abiotic surfaces in CFT073 and various UPEC strains. In addition, UpaG expression resulted in the adhesion of CFT073 to human bladder epithelial cells, with specific affinity to fibronectin and laminin. Prevalence analysis revealed that upaG is strongly associated with E. coli strains from the B2 and D phylogenetic groups, while deletion ofupaG had no significant effect on the ability of CFT073 to colonize the mouse urinary tract. Thus, UpaG is a novel trimeric AT adhesin from E. coli that mediates aggregation, biofilm formation, and adhesion to various ECM proteins.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Valle, Jaione" sort="Valle, Jaione" uniqKey="Valle J" first="Jaione" last="Valle">Jaione Valle</name></region><name sortKey="Beloin, Christophe" sort="Beloin, Christophe" uniqKey="Beloin C" first="Christophe" last="Beloin">Christophe Beloin</name><name sortKey="Ghigo, Jean Marc" sort="Ghigo, Jean Marc" uniqKey="Ghigo J" first="Jean-Marc" last="Ghigo">Jean-Marc Ghigo</name><name sortKey="Toledo Arana, Alejandro" sort="Toledo Arana, Alejandro" uniqKey="Toledo Arana A" first="Alejandro" last="Toledo-Arana">Alejandro Toledo-Arana</name><name sortKey="Wecker, Karine" sort="Wecker, Karine" uniqKey="Wecker K" first="Karine" last="Wecker">Karine Wecker</name></country><country name="Australie"><noRegion><name sortKey="Mabbett, Amanda N" sort="Mabbett, Amanda N" uniqKey="Mabbett A" first="Amanda N." last="Mabbett">Amanda N. Mabbett</name></noRegion><name sortKey="Schembri, Mark A" sort="Schembri, Mark A" uniqKey="Schembri M" first="Mark A." last="Schembri">Mark A. Schembri</name><name sortKey="Totsika, Makrina" sort="Totsika, Makrina" uniqKey="Totsika M" first="Makrina" last="Totsika">Makrina Totsika</name><name sortKey="Ulett, Glen C" sort="Ulett, Glen C" uniqKey="Ulett G" first="Glen C." last="Ulett">Glen C. Ulett</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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